RUNS LOCALLY IN YOUR BROWSER · NO FILES UPLOADED TO A SERVER
Raw Ct Data
Paste Sample, Gene, and Ct columns (tab-separated) — matching a typical instrument export. Use "Undetermined" or leave blank for no-amplification wells; multiple rows per sample+gene are treated as technical replicates and averaged.
Samples & Groups
Assign each sample to an experimental group, and choose which group is the calibrator (reference/control) that fold changes are computed relative to.
Gene Roles
Mark each gene as a target transcript or a reference/housekeeping gene. Multiple reference genes are averaged (arithmetic mean of Ct, equivalent to the geometric mean of quantity) for normalization.
Per-Sample ΔCt
Group Summary & Fold Change
Paste your Ct data above to begin.
Calculation Detail
Every number above, traced back to the raw Ct values, so the pipeline can be audited step by step.